Quality Control of ELISA
Quality Control of ELISA To help us to avoid the interference and variables, as the general this is the aim to reduce the errors Definition of interference :- *Defined as the effects of substances present in an analytical system which causes a deviation (variable value) of the measured values from the true value. *Assay interference can be analyte dependent or analyte independent. *May be increase or decrease the measured result. *The increasing (positive interference) which lacks of specificity while the decreasing (negative interference) which lacks of sensitivity. #We can put the errors and interferences as the following (must be avoid and correct these errors) :- 1. Pre analytical errors 2. Analytical errors 3. Post analytical errors Pre analytical errors :- As the following:- 1.Patient based:- *Incorrect sampling time. *Environment factors (may be changed analyte concentration and interpretation). 2.Specimen based:- *Blood collection procedure and tim...